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Health Cabinet Secretary James Macharia dismissed claims that one of the four patients tested positive for Ebola at Kenyatta National Hospital.
"I wish to assure Kenyans that the Ebola fever is not in Kenya. All the four patients who were admitted at the Kenyatta National Hospital with Ebola like symptoms have tested negative," Macharia told journalists.
He said a second test confirmed the earlier results that the four patients tested negative.
The government will suspend the entry of passengers travelling from Liberia, Guinea and Sierra Leone from Wednesday, as a measure to deal with the Ebola crisis.
The national carrier Kenya Airways will also suspend their commercial flight operations to the Ebola-hit region temporarily with effect from Wednesday in a bid to curb the spread of the fever into the country.
The World Health Organization declared the epidemic an international health emergency appealing for global aid.
Ebola, which spreads through the mucus and other body fluids or secretions such as stool, urine, saliva and semen of infected people, is believed to be very difficult to control.
It has killed more than 1,000 people in West African countries since earlier this year.